Fig. 3. Cell morphology and ACTB mRNA expression for different culture times. (A) Imaging of cell morphology (bright field) and ACTB mRNA by in situ RCA (dark field). Scale bars, 25 μm. (B) The average expression level and coefficient of variation for ACTB mRNA with different incubation times. (C) Single-cell fluctuation profiles for ACTB mRNA with different incubation times (n > 100, C.V. = s.d./mean).