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Average (mean) read yield in run 1 (n = 5 individuals). Multiplex reactions were performed in A with eight loci (proC10, proC18, proC22, proC36, proC37, proC44, proC48, proC49), in B with four loci (proC18, proC36, proC37, proC44), and in C with two loci (proC48 and proC49). Monoplex reactions were performed in D (proC10), E (proC18), F (proC36), G (proC48), and H (proC49). Error bars are standard deviation. MiSeq Reagent Kit v2 1 M reads was used.
