Figure 2.

OsNAC14 overexpression in rice enhances drought resistance. (A,B) Relative expression levels of OsNAC14 in non-transgenic (NT) and three independent T4 homozygous lines of PGD1::OsNAC14 (OsNAC14^OX) plants. Total RNAs were extracted from leaves (A) and roots (B) of two-week-old rice seedlings. OsUbi1 was used as internal control for normalization. Data represent mean value + SD (n = 3). (C) Measurement of soil moisture contents (mV). Data represent mean value ± SD of 30 measurements performed at different locations of soil. (D) The phenotype of transgenic rice plants during drought stress. Five-week-old three independent T4 homozygous lines of OsNAC14^OX plants and NT were exposed to drought stress for 3 days, followed by re-watering. Numbers on the image indicate duration of drought treatment and re-watering. (E) The survival rate of transgenic plants 5 days after re-watering. Data represent mean value + SD (n = 30). (F) Chlorophyll fluorescence (Fv/Fm) contents of plants under drought condition. Five-week-old three independent T4 homozygous lines of OsNAC14^OX and NT plants were exposed to drought stress for 11 days. Chlorophyll fluorescence was measured in the dark at indicated time point using a Pulse Amplitude Modulation (PAM) fluorometer. Data represent mean value + SD (n = 20). Significant differences from NT control are indicated by asterisks (one-tailed Student's t-test, ^*P < 0.05).