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. 2018 Jan 26;18(2):355. doi: 10.3390/s18020355

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FND uptake in HeLa cells. After confocal imaging, cells are analysed using FIJI software. Our analysis counts the objects and particles inside cells, giving an arbitrary measure for the amount of diamonds taken up. Objects are adjacent FND positive pixels in cells, incorporating both single particles and aggregates or adjacent particles. Particles represent an estimation of the amount of FNDs, calculated by the intensity and size of the objects. The sample incubated with more nanodiamonds, 10 µg of 70 nm FNDs T = 24, resulted in significantly more nanodiamonds per cell (p < 0.001) in comparison to most other samples with the exception of 10 µg of 70 nm FNDs T = 0.