Figure 2.

Morphometric analysis of lung sections from ApoE^−/− mice fed rodent chow without (Chow) or with second hand smoke exposure (Chow + SHS) or fed Western Diet without (WD) or with smoke exposure (WD + SHS). Representative image of hematoxylin and eosin stained lung tissue section from ApoE^−/− mice (Air spaces—A and Ductal spaces—D) (a). The MLI calculated using hematoxylin and eosin stained lung sections (b). Groups exposed to SHS scored significantly higher. For airspace group scores the Kruskal–Wallis ANOVA on ranks was used to assess statistical significance (p < 0.001) followed by post hoc testing with Dunn’s multiple comparison test for Chow ± SHS groups (p < 0.05). As Dunn’s test did not give a p value for the pairwise comparison of WD ± SHS groups, the non-parametric Mann-Whitney test with Bonferroni correction for multiple comparisons was used instead (p = 0.016). For ductal space group scores one way ANOVA was used (p < 0.001) followed by post hoc Fisher’s least significant difference tests. Chow ± SHS (p < 0.001), WD ± SHS (p = 0.004), three to five mice per group were evaluated. * Significant difference detected.