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Levels of MMP-9 in lung homogenates (a). Western blot from ApoE−/− mice fed WD and exposed to SHS only (WD + SHS), with Btk inhibitor treatment (WD + SHS/Btk Inh.), or treated with endothelial cell targeted siRNA for MMP-9 (WD + SHS/MECA-32 siRNA MMP-9). Three mice per group were analyzed (b). Western Blot from ApoE−/− mice fed WD and exposed to SHS only (WD + SHS) or treated with neutrophil targeted siRNA (WD + SHS/Ly6G siRNA MMP-9). Four animals per group were analyzed.
