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. 2018 Jan 25;19(2):353. doi: 10.3390/ijms19020353

Figure 4.

Figure 4

Figure 4

Dose and time dependence of toxic effects induced by CGS7181 and CGS7184. (A) HT22 cells were treated with potassium channel openers CGS7181 (diamonds) or CGS7184 (triangles). Cell survival was measured using the MTT assay 18 h after opener application. (B) HT22 cells were treated with the potassium channel opener CGS7184 at different concentrations: 30 µM (diamonds), 10 µM (circles), 3 µM (triangles), and 0.3 µM (squares). Cell survival was measured using the MTT assay 3, 6, 9, 12, and 18 h after opener application. (C) HT22 cells were treated with 3 µM CGS7184 in the presence of inhibitors of the BKCa channel. Cell survival was measured using the MTT assay 18 h after opener application. chtx-charybdotoxin (500 nM), ibtx-iberiotoxin (100 nM), pax-paxilline (2 µM). All data are expressed as means ± SEM from three independent experiments with three replicates per data point. # p < 0.05 vs. 3 µM CGS7184 and *** p < 0.001 vs. control by one-way ANOVA followed by Tukey’s test.