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. 2018 Jan 25;19(2):354. doi: 10.3390/ijms19020354

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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CytoViva dark-field images (400×) using Particle Fitting Function to locate MWCNT in lung tissue following a three-day particle exposure: (A) no particle dispersion media control; (B) source MWCNTs isolated mainly in alveolar macrophages. (C) carboxylated MWCNT_20.6 (20 min) starting to show some distribution in the lung lining in addition to AM cell collection; and (D) maximally carboxylated MWCNT_14.7 (120 min) demonstrates particle dispersion throughout the lung lining indicating epithelial cell involvement.