Table 2.

Kinetic affinity constants for the interactions of neo-glycoconjugates 9–16 with immobilized Gal-3-AVI.

Compound	Attached Glycan a	ka (M−1·s−1)	kd (s−1)	KD (M)
9	LacdiNAc	N.D.	N.D.	N.D.
10	LacNAc	N.D.	N.D.	N.D.
11	LacdiNAc-LacNAc	(5.9 ± 1.3) × 106	(8.5 ± 2.5) × 10−5	(1.4 ± 0.4) × 10−11
12	LacNAc-LacNAc	(6.2 ± 2.1) × 106	(4.2 ± 3.1) × 10−4	(6.8 ± 3.9) × 10−11
13	LacdiNAc-LacNAc-LacNAc	(5.8 ± 2.2) × 106	(1.5 ± 0.4) × 10−4	(2.6 ± 0.9) × 10−11
14	LacNAc-LacNAc-LacNAc	(7.8 ± 2.9) × 106	(5.1 ± 2.8) × 10−4	(6.5 ± 2.6) × 10−11
15	LacNAc type 1-LacNAc-LacNAc	(4.9 ± 1.8) × 106	(1.3 ± 0.3) × 10−3	(2.7 ± 1.0) × 10−10
16	Galili-LacNAc-LacNAc	(9.3 ± 3.1) × 106	(6.3 ± 2.0) × 10−4	(6.8 ± 3.5) × 10−11
ASF	Positive control standard	(4.8 ± 1.9) × 104	(4.0 ± 1.7) × 10−4	(8.3 ± 3.1) × 10−9

^a Structures of glycans attached to respective neo-glycoproteins are depicted in Figure 1. N.D., not determined.