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. 2018 Jan 27;19(2):382. doi: 10.3390/ijms19020382

Figure 4.

Figure 4

In vitro phospho-signaling analysis of primary AML cells derived from five patients to explore the effects of indomethacin on the PI3K-Akt-mTOR pathway. AML cells were incubated in medium alone, in medium supplemented with 10 μg/mL of either indomethacin or insulin, and in medium supplemented with the combination of insulin and indomethacin. Phosphorylation status of nine mediators were examined. An indomethacin-induced decrease of mTOR pS2448, S6 pS235 pS236, and S6 pS244 was seen for all patients in insulin-free and/or insulin-supplemented cultures, and a decrease of S6 pS240 and Akt pS473 was seen for four of the five patients. The X-axis is a log-scale for fluorescence intensity; the Y-axis indicates the number of cells.