Figure 5.

The effect of indomethacin on the activation of PI3K-Akt-mTOR signaling. We investigated the effects of indomethacin on PI3K-Akt-mTOR signaling in primary AML cells derived from five patients. For each sample, we tested AML cells incubated in medium alone, with only indomethacin 10 μg/mL, in medium supplemented with 10 μg/mL insulin, and with the combination of insulin and indomethacin. Phosphorylation status of nine mediators were examined. Red indicates high and blue indicates low phosphorylation/expression of the mediators. All combinations tested for each patient sample also clustered together in the same subclusters for all patients. All values from the flow cytometric analyses were calculated using fold change on the Inverse hyperbolic sine (Arcsinh) scale.