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. 2018 Jan 27;19(2):384. doi: 10.3390/ijms19020384

Figure 1.

Figure 1

Distributional changes of anti-cofilin and phalloidin rhodamine in DRG growth cones subsequent to application of NGF, VEGF, or BMS-5. Controls (a) show a congruent distribution pattern of cofilin (green) and actin (red) (n = 51). Stimulation with NGF (b) (n = 53) or VEGF (c) (n = 69) lead to a highly significant increase in their mean circumference and area for both actin and cofilin, compared to control conditions (*** = p < 0.0001). The number of filopodia increased. Inhibition of cofilin signaling by application of the LIMK-inhibitor BMS-5 (d) under control conditions (n = 55) and (e) under stimulation with VEGF plus NGF (n = 57) did not lead to significant alterations of growth cone morphology as compared to controls. The table (f) shows statistical data of anti-cofilin-stained growth cones. The graphs show the mean circumference (g) and the mean area (h) of the analyzed growth cones. Scale bars = 10 µm. Error bars = SEM.