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. 2018 Jan 29;19(2):391. doi: 10.3390/ijms19020391

Table 1.

Primer sequences used for quantification of Sphingosine-1-phosphate (S1P) receptor subtypes and β2-microglobulin.

Primer Sequence
S1P1 forward 5′-AAC TTT GCG AGT GAG CTG GT-3′
S1P1 reverse 5′-CTA GAG GGC GAG GTT GAG TG-3′
S1P2 forward 5′-ATA GAC CGA GCA CAG CCA AC-3′
S1P2 reverse 5′-GAG GTG GTC TCC TGC ATG TC-3′
S1P3 forward 5′-AAG CCT AGC GGG AGA GAA AC-3′
S1P3 reverse 5′-TCA GGG AAC AAT TGG GAG AG-3′
S1P4 forward 5′-GGA CTT CTC GGT CAC TCA GC-3′
S1P4 reverse 5′-GGC TTG CTG TCA TGT TCT CA-3′
S1P5 forward 5′-GGA GGG ACT CTC CTG GAT TC-3′
S1P5 reverse 5′-TTC CTC TGT AGC CAG CCA CT-3′
β2 microglobulin forward 5′-ATT CAC CCC CAC TGA GAC TG-3′
β2 microglobulin reverse 5′-GCT ATT TCT TTC TGC GTG CAT-3′