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WSPIS disturbed cell cycle progression and caused DNA damage. (A,B) DNA content analysis. THP-1 and U937 cells were treated with various doses of WSPIS (100, 250 and 500 µg/mL) for 24 and 48 h. DNA content was analyzed using flow cytometry after propidium iodide staining. (C) The protein level of γ-H2AX was determined by Western blotting in WSPIS-treated THP-1 cells. DNA content histograms and Western blotting data are representative of three independent experiments.
