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. 2018 Jan 29;19(2):393. doi: 10.3390/ijms19020393

Figure 6.

Figure 6

Effect of N-acetyl-l-cysteine (NAC) on WSPIS-induced DNA damage and apoptosis in THP-1 cells. Cells were pretreated with NAC (1 mM) for 1 h followed by WSPIS treatment for the indicated time periods. (A) Intracellular ROS content was evaluated at 1 h after 500 μg/mL WSPIS treatment by measuring oxidized DCF fluorescence using flow cytometry. (B) The protein level of γ-H2AX was determined by Western blotting at 48 h after WSPIS treatment. (C) Oligonucleosomal DNA fragmentation was analyzed by agarose gel electrophoresis at 48 h after WSPIS treatment. (D) Cell viability was determined by the trypan blue exclusion assay at 24 and 48 h after WSPIS treatment. Values are expressed as a percentage compared to the control. Data are representative of three independent experiments.