Table 2.
qRT-PCR Primer.
| Gene | Accession No. | Primer Sequence |
|---|---|---|
| 18S rRNA | NM_022551 | Forward: 5′ CGGAAAATAGCCTTTGCCATC 3′ |
| Reverse: 5′ AGTTCTCCCGCCCTCTTGGT 3′ | ||
| COL1A1 | NM_000088.3 | Forward: 5′ TGACCTCAAGATGTGCCACT 3′ |
| Reverse: 5′ ACCAGACATGCCTCTTGTCC 3′ | ||
| COL3A1 | NM_000090.3 | Forward: 5′ GCTGGCATCAAAGGACATCG 3′ |
| Reverse: 5′ TGTTACCTCGAGGCCCTGGT 3′ | ||
| COL5A2 | NM_000393 | Forward: 5′ AGAAGCCTCCCAGAACATCA 3′ |
| Reverse: 5′ ACAGTCTTGCCCACATTTCC 3′ | ||
| MMP1 | NM_002421.3 | Forward: 5′ CACGCCAGATTTGCCAAGAG 3′ |
| Reverse: 5′ GTCCCGATGATCTCCCCTGA 3′ | ||
| MMP2 | NM_ 004530 | Forward: 5′ TGGATGATGCCTTTGCTCGT 3′ |
| Reverse: 5′ CCAGGAGTCCGTCCTTACCG 3′ | ||
| MMP3 | NM_002422.3 | Forward: 5′ TGGGCCAGGGATTAATGGAG 3′ |
| Reverse: 5′ GGCCAATTTCATGAGCAGCA 3′ | ||
| MMP9 | NM_004994.2 | Forward: 5′ GGGACGCAGACATCGTCATC 3′ |
| Reverse: 5′ GGGACCACAACTCGTCATCG 3′ | ||
| MMP10 | NM_ 002425 | Forward: 5′ CCACCTGGACCTGGGCTTTA 3′ |
| Reverse: 5′ GAACTGGGCGAGCTCTGTGA 3′ | ||
| MMP13 | NM_002427.3 | Forward: 5′ CCTTCCCAGTGGTGGTGATG 3′ |
| Reverse: 5′ CGGAGCCTCTCAGTCATGGA 3′ | ||
| TIMP1 | NM_003254.2 | Forward: 5′ TTGGCTGTGAGGAATGCACA 3′ |
| Reverse: 5′ AAGGTGACGGGACTGGAAGC 3′ | ||
| TIMP2 | NM_003255.4 | Forward: 5′ CCTGAGCACCACCCAGAAGA 3′ |
| Reverse: 5′ TCCATCCAGAGGCACTCGTC 3′ | ||
| TIMP3 | NM_000362.4 | Forward: 5′ CCGAGGCTTCACCAAGATGC 3′ |
| Reverse: 5′ GCCATCATAGACGCGACCTG 3′ | ||
| TIMP4 | NM_003256.3 | Forward: 5′ GAAGCCAACAGCCAGAAGCA 3′ |
| Reverse: 5′ TTCCCTCTGCACCAAGGACA 3′ | ||
| TNMD | NM_022144.2 | Forward: 5′ TTGAAGACCCACGAAGTAGA 3′ |
| Reverse: 5′ ATGACATGGAGCACACTTTC 3′ | ||
| IL33 | NM_033439 | Forward: 5′ CCAACAGAAGGCCAAAGAAG 3′ |
| Reverse: 5′ AAGGCAAAGCACTCCACAGT 3′ | ||
| sST2 | NM_003856 | Forward: 5′ CAACAAGAGGAAGGGCAAAA 3′ |
| Reverse: 5′ CAAATTCAGGGCCAGACAGT 3′ | ||
| IL1β | NM_000576 | Forward: 5′ TCCAGGAGAATGACCTGAGC 3′ |
| Reverse: 5′ GTGATCGTACAGGTGCATCG 3′ | ||
| TNFα | NM_000594 | Forward: 5′ AGCCCATGTTGTAGCAAACC 3′ |
| Reverse: 5′ GAGGTACAGGCCCTCTGATG 3′ | ||
| IL6 | NM_000600 | Forward: 5′ TGAGGAGACTTGCCTGGTGA 3′ |
| Reverse: 5′ TTGGGTCAGGGGTGGTTATT 3′ | ||
| IL10 | NM_000572 | Forward: 5′ TGAGAACAGCTGCACCCACT 3′ |
| Reverse: 5′ GGCAACCCAGGTAACCCTTA 3′ | ||
| TGFβ1 | NM_000660.4 | Forward: 5′ AAGGACCTCGGCTGGAAGTG 3′ |
| Reverse: 5′ AGGGCCAGGACCTTGCTGTA 3′ | ||
| CD3 | NM_000073 | Forward: 5′ CTGGGAAGTAATGCCAAGGA 3′ |
| Reverse: 5′ CCAACAGCAAGGACGAAAAT 3′ | ||
| CD68 | NM_001040059 | Forward: 5′ CCACCTGCTTCTCTCATTCC 3′ |
| Reverse: 5′ ATTGTACTCCACCGCCATGT 3′ | ||
| CD80 | NM_005191 | Forward: 5′ GCAGGGAACATCACCATCCA 3′ |
| Reverse: 5′ CAGGACAGCGTTGCCACTTC 3′ | ||
| CD206 | NM_002438 | Forward: 5′ ACTGGGGCCAAGCTTCTCTG 3′ |
| Reverse: 5′ CACAGCCACGTCCCTTCAAC 3′ | ||
| MIF | NM_002415 | Forward: 5′ GGTTCCTCTCCGAGCTCACC 3′ |
| Reverse: 5′ TAGACCCTGTCCGGGCTGAT 3′ | ||
| PGP9.5 | NM_004181 | Forward: 5′ CCATACAGGCAGCCCATGAT 3′ |
| Reverse: 5′ AGACCTTGGCAGCGTCCTTC 3′ | ||
| GAP43 | NM_002045 | Forward: 5′ CCGGCAAAGCAGGAGAAACT 3′ |
| Reverse: 5′ TGGAGGACGGCGAGTTATCA 3′ | ||
| COX2 | NM_000963 | Forward: 5′ TAGAGCCCTTCCTCCTGTGC 3′ |
| Reverse: 5′ TGGGGATCAGGGATGAACTT 3′ | ||
| FABP4 | NM_001442 | Forward: 5′ ACTGGGCCAGGAATTTGACG 3′ |
| Reverse: 5′ ATGACGCATTCCACCACCAG 3′ | ||
| PPARγ | NM_015869 | Forward: 5′ AAAGTCCTTCCCGCTGACCA 3′ |
| Reverse: 5′ GGCCACCTCTTTGCTCTGCT 3′ | ||
| ADIPOQ | NM_004797 | Forward: 5′ TGACCAGGAAACCACGACTCA 3′ |
| Reverse: 5′ CCGATGTCTCCCTTAGGACCA 3′ | ||
| CEBPα | NM_004364 | Forward: 5′ AAGGCCAAGAAGTCGGTGGA 3′ |
| Reverse: 5′ GGCGGTCATTGTCACTGGTC 3′ | ||
| SCX | Commercial Quantitect primer Assay Hs_SCXB_2_SG | |