Figure 2.

Cytokine profile of media collected from luminal (upper) and abluminal (lower) compartments. Media was collected from luminal (insert, upper) and abluminal (24-well plate, lower) compartments separated by a porous membrane at 5 DIV after exposure to FBS, plasma from ALS or control patient. At luminal side, significant increase of IFN-γ, TNF-α, IL-5, and IL-6 cytokine concentrations were determined at luminal compartment primarily after cell exposure to ALS plasma. There were no differences in cytokine levels between any cell culture conditions at abluminal side of construct. Data are presented as means ± S.E.M. * p < 0.05, ** p < 0.01, *** p < 0.001.