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. 2018 Feb 2;19(2):447. doi: 10.3390/ijms19020447

Figure 1.

Figure 1

Luteolin (LTL) inhibits proliferation of and induces apoptosis in A549 and H460 cancer cells. (A) Cell proliferation was measured with a cell counting kit-8 (CCK-8) assay when A549 and H460 cancer cells were treated with LTL at various concentrations (5, 10, 20, 30, 40, 60, 80, and 100 μM) for up to 72 h; (B) Apoptosis of A549 and H460 cells was quantified 24 h after treatment with LTL at various concentrations (10, 20, and 40 µM) by Annexin V-Fluorescein (FITC)/Propidium Iodide (PI) double-staining; (C) Bar graphs represent the proportion of early and late stage apoptosis cells. The results are expressed as means ±Standard Deviation (SD) of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the controls.