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. 2018 Feb 6;19(2):472. doi: 10.3390/ijms19020472

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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A potential bidirectional regulation loop between HOTTIP/HOXA11 in MCF-7 cells. (A): Expression of HOXA genes under HOTTIP knockdown in MCF-7 cells. (B): The effect of HOTTIP knockdown on HOXA11 expression at the protein level. (C): Screening of HOXA11 shRNA sequences by RT-qPCR. (D): HOXA11 knockdown decreased the expression of HOTTIP by RT-qPCR. (E): Rescue experiment of HOXA11 downregulation by HOTTIP knockdown by RT-qPCR. (F): Rescue experiment of HOXA11 downregulation by HOTTIP knockdown by Western blot. * p < 0.05.