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. 2018 Feb 6;19(2):475. doi: 10.3390/ijms19020475

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Osteogenic differentiation of osteoprogenitors from osteoarthritic subchondral bone. (a) Visualization of ALP activity in osteoprogenitors cultured in complete and osteogenic medium for three weeks. Quantification of ALP enzymatic activity in (b) complete and (c) osteogenic medium. Data are presented as scatter dotplot with means + SEM. (d) Evaluation of matrix mineralization (red) in osteoprogenitors from osteoarthritic knee and hip joints by alizarin red staining. NS: non-sclerotic, SC: sclerotic, IC: iliac crest, CM: complete medium, OM: osteogenic medium.