Table 4.

Th17 CpGs showing statistically significant changes (p ≤ 0.05) during first and second halves of pregnancy ^a.

Th17 Gene Names	Total Number of CpGs	Significant CpGs in First Half of Pregnancy b					Significant CpGs in Second Half of Pregnancy c
		No. of Sign. CpGs	CpG Sites	Parameter Estimates	p-Value	FDR-Adjusted p-Value	No. of Sign. CpGs	CpG Sites	Parameter Estimates	p-Value	FDR-Adjusted p-Value
IL17A	2	1	cg05884768	−0.21	0.046	0.155	0
IL17B	7	1	cg01579636	0.21	0.002	0.014	2	cg01579636	0.13	0.011	0.047
								cg05860978	−0.12	0.014	0.059
IL17C	5	3	cg08155347	−0.21	0.012	0.058	2
			cg26686608	0.14	3.27 × 10−4	0.003		cg26686608	0.14	0.009	0.042
			cg27132152	0.18	0.009	0.050		cg27132152	0.23	0.004	0.020
IL17D	13	1	cg09985351	0.2	0.047	0.155	2
								cg12475590	−0.27	0.002	0.017
								cg02792322	−0.17	0.006	0.031
IL17F	5	0					0
IL17RA	8	5	cg01085328	0.17	0.011	0.055	4
			cg01760983	−0.65	1.23 × 10−4	0.001		cg01760983	−0.24	0.039	0.130
			cg02866761	−0.16	0.001	0.009
			cg16389078	−0.52	2.72 × 10−8	<1.0 × 10−6		cg16389078	−0.36	0.001	0.005
			cg19901866	0.24	0.032	0.118
								cg13595439	0.15	0.015	0.061
								cg15502903	−0.19	1.76 × 10−4	0.002
IL17RC	2	0					0
IL17RD	15	4	cg00770158	−0.09	0.029	0.110	5
			cg01797381	−0.29	0.005	0.027		cg01797381	−0.32	4.18 × 10−4	0.004
			cg03435901	−0.48	5.82 × 10−5	0.001		cg03435901	−0.4	0.003	0.020
			cg10882522	−0.28	0.001	0.009		cg10882522	−0.18	0.019	0.073
								cg09429700	0.11	0.004	0.020
								cg00743540	0.19	0.002	0.014
IL17RE	8	4	cg02968508	−0.11	0.025	0.101	2
			cg05253480	0.2	0.017	0.070
			cg06619959	−0.16	0.003	0.016		cg06619959	−0.1	0.003	0.011
			cg15095327	0.13	0.043	0.151		cg15095327	0.19	0.031	0.116
IL17REL	12	5	cg00692279	0.12	4.28 × 10−4	0.004	4
			cg04485799	0.14	0.026	0.101		cg04485799	0.13	0.039	0.130
			cg12009803	0.11	0.013	0.061
			cg26206185	0.55	2.3 × 10−5	3.5 × 10−5
			cg27068297	−0.14	0.003	0.016
								cg00090674	0.23	2.16 × 10−4	0.002
								cg13563334	0.15	1.96 × 10−5	3.5 × 10−5
								cg00692279	0.13	0.007	0.032
IL21	3	1	cg00136405	−0.16	0.014	0.062	1	cg00136405	−0.16	0.004	0.020
IL21R	12	7	cg02656594	−0.53	2.86 × 10−14	<1.0 × 10−6	7	cg02656594	−0.52	2.89 × 10−15	<1.0 × 10−6
			cg00050618	0.26	0.001	0.006		cg00050618	0.22	3.05 × 10−6	6.0 × 10−6
			cg05814654	−0.86	5.46 × 10−6	1.2 × 10−5		cg05814654	−0.82	1.01 × 10−4	0.002
			cg02983090	−0.72	9.87 × 10−6	1.7 × 10−5		cg02983090	−0.73	2.14 × 10−7	1.0 × 10−6
			cg08282819	−1.07	5.33 × 10−9	<1.0 × 10−6		cg08282819	−0.96	4.29 × 10−14	<1.0 × 10−6
			cg19423311	−0.48	0.048	0.15494		cg19423311	−0.41	0.035	0.125
			cg27027151	−0.47	4.7 × 10−11	<1.0 × 10−6
IL22	8	0					1	cg13851647	−0.22	0.046	0.149
IL22RA1	4	1	cg21293216	0.28	0.009	0.050	3	cg21293216	0.4	1.55 × 10−4	0.002
								cg09152089	0.18	0.032	0.116
								cg11651446	0.17	0.002	0.014
IL22RA2	2	1	cg00415333	0.14	0.050	0.155	0
Total number	106	34					33

^a CpGs with statistically significant differences after applying false discovery tests are bolded; ^b Changes in methylation from age 18 years to first half of pregnancy (8–21 weeks) for Treg pathway gene CpGs; ^c Changes in methylation from age 18 years to second half of pregnancy (22–38 weeks) for Treg pathway gene CpGs.