Table 5.

Treg CpGs showing statistically significant changes (p ≤ 0.05) during first and second halves of pregnancy ^a.

Treg Gene Names	Total Number of CpGs	Significant CpGs in First Half of Pregnancy b					Significant CpGs in Second Half of Pregnancy c
		No. of Sign. CpGs	CpG Sites	Parameter Estimates	p-Value	FDR-Adjusted p-Value	No. of Sign. CpGs	CpG Sites	Parameter Estimates	p-Value	FDR-Adjusted p-Value
FOXP3	7	3	cg01905377	−1.01	2.19 × 10−8	<1.0 × 10−6	4	cg01905377	−1.28	7.46 × 10−11	<1.0 × 10−6
			cg06767008	0.42	7.46 × 10−12	<1.0 × 10−6		cg06767008	0.37	2.22 × 10−6	0.10
			cg15614573	−0.41	0.033	0.11		cg15614573	−0.81	0.001	1.0 × 10−5
								cg04920616	0.21	0.040	0.004
CTLA4	3	0					0
Total	10	3					4

^a CpGs with statistically significant differences after applying false discovery tests are bolded; ^b Changes in methylation from age 18 years to first half of pregnancy (8–21 weeks) for Treg pathway gene CpGs; ^c Changes in methylation from age 18 years to second half of pregnancy (22–38 weeks) for Treg pathway gene CpGs.