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Breadth of DNA-induced IFN-γ responses. Fresh PBMCs from volunteer BJ10 (Biojector i.m.), who expressed the alleles HLA-A*0101, -A*0201, -B*0701, -DRB1*1501, and -DRB5*0101, were cultured with (a) six class I-restricted PfCSP peptides, an HLA-A2.1-restricted positive control peptide (Flu. A2.1), or an HLA-A2.1-restricted negative control peptide (HIV gag A2.1); or (b) six class II-restricted PfCSP peptides or an HLA-DR restricted negative control peptide (P. falciparum Exp-1 DR).
