Table 1.
Primer Name | Primer Sequence (5′→3′) | Purpose |
---|---|---|
DsSWEET12-qF | CAAAGCATTCCCTATGTGGTG | qPCR |
DsSWEET12-qR | TAGTTTGCTTGTCGGCGTAGA | qPCR |
DsActin-qF | CGGTGGCTCTATCCTCGCTT | qPCR |
DsActin-qR | TTCCTGTGGACGATTGACGG | qPCR |
AtActin1-F (AT2G37620) | GAAAATGGCTGATGGTGAAG | RT-PCR |
AtActin1-R | CTCATAGATAGGAACAGTGTGGC | RT-PCR |
DsSWEET12 (BamHI)-F | GGATCCATGACTACTTTTGCTCACTTC | Cloning and Subcellular localization |
DsSWEET12 (SacI)-R | GAGCTCTTAGGCAGTCACGGCAATT | Cloning |
DsSWEET12 (KpnI)-R | GGTACCGCAGTCACGGCAATTTGAG | Subcellular localization |