Table 4.
Comparison of DNA extraction methods in five samples of cystic fibrosis patients with known A. fumigatus colonization.
| Subject | Sputum Galactomannan, ODI | PCR after EasyMAG Extraction, Ct | PCR after MycXtra® Extraction, Ct |
|---|---|---|---|
| CF1 | 4.4 | negative | positive, 36.7 |
| CF2 | 3.7 | negative | positive, 32.9 |
| CF3 | 4.7 | positive, 29.3 | positive, 26.2 |
| CF4 | 4.6 | positive, 30.7 | positive, 30.5 |
| CF5 | 4.7 | negative | positive, 33.2 |
Sputum galactomannan is presented as optical density index. PCR is expressed as negative or positive with the respective Ct value in case of positivity. CF: cystic fibrosis sample, ODI: optical density index, PCR: polymerase chain reaction, Ct: cycle threshold.