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. 2018 Feb 9;19(2):524. doi: 10.3390/ijms19020524

Figure 2.

Figure 2

Internally controlled multiplex TEC-LAMP detection. The four graphs show fluorescence recorded in the FAM (A), Cy5 (B), HEX (C) and CYAN (D) LightCycler® 480 detection channels, for three TEC-LAMP reactions performed in parallel. These reactions included: (blue) 100 genome copies S. pneumoniae, N. meningitidis and H. influenzae in the presence of 50 copies IAC; (red) no bacterial template in the presence of 50 copies IAC; and (black) a NTC reaction using molecular grade water in place of bacterial or IAC templates. Successful simultaneous detection of all three bacterial targets in the presence of the IAC was observed (blue: A–C). The two control reactions performed successfully as detection of the IAC in the absence of bacterial target was observed (Red: D), and no detection was observed in the NTC reaction (Black: A–D).