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. 2018 Feb 9;19(2):524. doi: 10.3390/ijms19020524

Table 1.

TEC-LAMP oligonucleotides.

Primer Type Sequence (5′-3′)
S. pneumoniae
TEC primer/probe (FAM)TGGA(dSpacer)AA(BHQ1-dT)GCTCTGGCTTTTGAAGTGA-CCTACACCAATATCCTCGCT
Forward Inner TGGAAAATGCTCTGGCTTTTGAAGTGA-CCTACACCAATATCCTCGCT
Reverse Inner TCTGTCTGGTAGACAGAATGACGGA-TCTTTGAGAATCAGATGCTGGA
Forward Outer TCCGTCAACGAGGCACAA
Reverse Outer AGCAAACTCACCAAGCGC
Forward Loop TGATGAAACAGACAAGCTGATTCT
Reverse Loop GCGCAATGATGGTATAATCCAG
N. meningitidis
TEC primer/probe (Cy5)TGTC(dSpacer)G(BHQ2-dT)GGCTTTGTTGGTGGTGTCGC-GTGCAAACAGATACGTCCG
Forward Inner TGTCGGTGGCTTTGTTGGTGGTGTCGC-GTGCAAACAGATACGTCCG
Reverse Inner CCGATGTACCAGCACCTTGTCC-GTTTGCGCTGATTACGCCTC
Forward Outer CCCAATTCCACATCAATACGTG
Reverse Outer GTGGTGTCGGTGGTGTTG
Forward Loop GAGATTGTGTTGGGCGGTTTG
Reverse Loop CACCACTTGGAAAAACAGAGGC
H. influenzae
TEC primer/probe (HEX)TGCC(dSpacer)C(BHQ1-dT)GCTTCACGTAAATTATTTGG-TGCTTATTCCTATCGTGGTACG
Forward Inner TGCCGCTGCTTCACGTAAATTATTTGG-TGCTTATTCCTATCGTGGTACG
Reverse Inner CTTGGTTGCTCTCAATGGCAAG-GCACGCCAGTTAAAATCCCT
Forward Outer GGCTGGAGCATTCGCATT
Reverse Outer TTCTCCTGAAATTCGGGCAA
Forward Loop AACATATTGTCCGTAGTGCG
Reverse Loop TGATGATTTGTTATCGAGCAGC
Internal Amplification Control
TEC primer/probe (CYAN)TGTT(dSpacer)A(BHQ1-dT)ATCCGCGATCCTTGCGTTGT-TCCCCGCTATGGAAGGTC
Forward Inner TGTTTATATCCGCGATCCTTGCGTTGT-TCCCCGCTATGGAAGGTC
Reverse Inner CACCTGTTCGTGTCGTATCGGT-ATGCATTACCAGAGTGCTCC
Forward Outer TACAGCGAAAAGCCCAGC
Reverse Outer AAGCGACGAATGTCCTGTG
Forward Loop TCTTAATTGCTTGCCGGAGC
Reverse Loop GAGCCATGTGCCATACTCGTC

FAM, 6-carboxyfluorescein fluorophore; dSpacer, 1′,2′-dideoxyribose; BHQ1-dT, black hole quencher 1 linked to thymine; -, separation between 5′ antisense and 3′ sense inner primer sequences; Cy5, cyanine fluorophore; BHQ2-dT, black hole quencher 2 linked to thymine; HEX, 6-hexachlorofluorescein fluorophore; CYAN, LC®CYAN 500 fluorophore.