Table 1.
Primer Type | Sequence (5′-3′) |
---|---|
S. pneumoniae | |
TEC primer/probe | (FAM)TGGA(dSpacer)AA(BHQ1-dT)GCTCTGGCTTTTGAAGTGA-CCTACACCAATATCCTCGCT |
Forward Inner | TGGAAAATGCTCTGGCTTTTGAAGTGA-CCTACACCAATATCCTCGCT |
Reverse Inner | TCTGTCTGGTAGACAGAATGACGGA-TCTTTGAGAATCAGATGCTGGA |
Forward Outer | TCCGTCAACGAGGCACAA |
Reverse Outer | AGCAAACTCACCAAGCGC |
Forward Loop | TGATGAAACAGACAAGCTGATTCT |
Reverse Loop | GCGCAATGATGGTATAATCCAG |
N. meningitidis | |
TEC primer/probe | (Cy5)TGTC(dSpacer)G(BHQ2-dT)GGCTTTGTTGGTGGTGTCGC-GTGCAAACAGATACGTCCG |
Forward Inner | TGTCGGTGGCTTTGTTGGTGGTGTCGC-GTGCAAACAGATACGTCCG |
Reverse Inner | CCGATGTACCAGCACCTTGTCC-GTTTGCGCTGATTACGCCTC |
Forward Outer | CCCAATTCCACATCAATACGTG |
Reverse Outer | GTGGTGTCGGTGGTGTTG |
Forward Loop | GAGATTGTGTTGGGCGGTTTG |
Reverse Loop | CACCACTTGGAAAAACAGAGGC |
H. influenzae | |
TEC primer/probe | (HEX)TGCC(dSpacer)C(BHQ1-dT)GCTTCACGTAAATTATTTGG-TGCTTATTCCTATCGTGGTACG |
Forward Inner | TGCCGCTGCTTCACGTAAATTATTTGG-TGCTTATTCCTATCGTGGTACG |
Reverse Inner | CTTGGTTGCTCTCAATGGCAAG-GCACGCCAGTTAAAATCCCT |
Forward Outer | GGCTGGAGCATTCGCATT |
Reverse Outer | TTCTCCTGAAATTCGGGCAA |
Forward Loop | AACATATTGTCCGTAGTGCG |
Reverse Loop | TGATGATTTGTTATCGAGCAGC |
Internal Amplification Control | |
TEC primer/probe | (CYAN)TGTT(dSpacer)A(BHQ1-dT)ATCCGCGATCCTTGCGTTGT-TCCCCGCTATGGAAGGTC |
Forward Inner | TGTTTATATCCGCGATCCTTGCGTTGT-TCCCCGCTATGGAAGGTC |
Reverse Inner | CACCTGTTCGTGTCGTATCGGT-ATGCATTACCAGAGTGCTCC |
Forward Outer | TACAGCGAAAAGCCCAGC |
Reverse Outer | AAGCGACGAATGTCCTGTG |
Forward Loop | TCTTAATTGCTTGCCGGAGC |
Reverse Loop | GAGCCATGTGCCATACTCGTC |
FAM, 6-carboxyfluorescein fluorophore; dSpacer, 1′,2′-dideoxyribose; BHQ1-dT, black hole quencher 1 linked to thymine; -, separation between 5′ antisense and 3′ sense inner primer sequences; Cy5, cyanine fluorophore; BHQ2-dT, black hole quencher 2 linked to thymine; HEX, 6-hexachlorofluorescein fluorophore; CYAN, LC®CYAN 500 fluorophore.