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. 2018 Feb 10;19(2):537. doi: 10.3390/ijms19020537

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Osteogenic, adipogenic, and chondrogenic differentiations of equine umbilical cord blood-derived mesenchymal stem cells (eUCB-MSCs). (A) Differentiations were performed at passage four after amplification without FGF-2; (B) Differentiations were performed at passage four after amplification with FGF-2; (C) Success rate for the differentiation of eUCB-MSCs into osteoblasts, adipocytes, and chondrocytes (n = 10). After culture in osteoblastic induction medium, calcium mineralization was demonstrated by Alizarin Red S staining (magnification ×10). After adipogenic induction and incubation in the maintenance medium, no lipid droplets in the cytoplasm were observed with Oil Red O staining (magnification ×10). After chondrogenic induction, sulfated proteoglycans of the neo-synthetisized matrix were demonstrated by Alcian blue staining (magnification ×20).