Table 3.

HPV DNA detection by multiplex PCR.

PCR Primer Mixture		Run	Number of Tested Samples
Mixture 1	HPV16, 18, 31, 45	1	55
		2	55
Duplex only	HPV16 and β-globin	1	55
		2	30
		3	30
Mixture 1 and 2	HPV16, 18, 31, 45 and HPV33, 52, 58 and β-globin	1	15