Table 1.

Mutations introduced to the TROL presequence by the QuikChange Multi Site-Directed Mutagenesis and corresponding primer sequences.

Change	Mutation/Primer Name	Primer Sequence
e1	g199a_c200t	aagcagtgcaacagctcctattaaatccctgacgtacgag
e2	c212a_g213t	gctcctgctaaatccctgaattacgaggaagctctgcaac
e3	t214g_a215t	ctgctaaatccctgacggtcgaggaagctctgcaac
e4	g217c_g220c	gctaaatccctgacgtaccagcaagctctgcaacaatcta
e5	c226a_t227c	ccctgacgtacgaggaagctacgcaacaatctatgacca
e6	c232g_a233t	cgtacgaggaagctctgcaagtatctatgaccacttcttca
	TROLfor	caccatggaagctctgaaaaccgca
	TROLrev	gggctgcgatggcatcg