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. 2018 Feb 16;19(2):585. doi: 10.3390/ijms19020585

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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OPN distribution in fractions obtained with ceramic hydroxyapatite (CHT) resin (a) with no salt; (b) with 250 mM NaCl. Step elutions were performed with sodium phosphate (NaP) buffer, pH 6.8. Data (% OPN) were generated by ImageJ analysis of anti-FLAG western blots and normalized by the amount in the lysate. Error bars indicate standard deviation of triplicate ImageJ measurements.

OPN distribution in fractions obtained with ceramic hydroxyapatite (CHT) resin (a) with no salt; (b) with 250 mM NaCl. Step elutions were performed with sodium phosphate (NaP) buffer, pH 6.8. Data (% OPN) were generated by ImageJ analysis of anti-FLAG western blots and normalized by the amount in the lysate. Error bars indicate standard deviation of triplicate ImageJ measurements.