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. 2018 Feb 16;19(2):593. doi: 10.3390/ijms19020593

Figure 1.

Figure 1

ApoERα regulated genes through SE binding. (a,b) Volcano plots reporting the adjusted p-value and the log2 Fold Change (FC) of (a) the protein-coding genes and (b) lncRNAs computed from RNA-seq data comparing MCF-7 cells transfected with siERα and siCTR in hormone-deprived (HD) medium; (c) Bar plot reporting the -log10 adjusted p-value of the most significant Gene Ontology Biological Processes enriched for the genes down-regulated (top) or up-regulated (down) upon ERα silencing in HD; (d) Heat map illustrating differential expression of Super-Enhancer (SE)-associated lncRNAs upon siERα transfection and upon nine-hours E2 treatment; (e) Heat map illustrating expression values of SE-associated lncRNAs as FPKM; (f) Bar plot reporting the log2 relative expression of ERα mRNA and eight candidate SE-associated lncRNAs upon ERα silencing in HD; p-value of three biological replicates by unpaired t-test: * p < 0.05; ** p < 0.01; *** p < 0.001; (g) Bar plot reporting the relative expression of eight candidate SE-associated lncRNAs and GREB1 (positive control) in a time-course experiment of 17β-estradiol (E2) or vehicle (Veh) treatment; SD of three biological replicates; p-value by unpaired t-test: * p < 0.05; ** p < 0.01; *** p < 0.001.