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. 2018 Feb 16;19(2):593. doi: 10.3390/ijms19020593

Figure 3.

Figure 3

Analysis of ERα co-factor binding at DSCAM-AS1 locus. (a,b) Bar plots reporting p300, GATA3 and FoxM1 fold enrichment over IgG signal at DSCAM-AS1 promoter (a) and E6-enhancer (b) by ChIP-qPCR in MCF-7 cells grown in HD and transfected with siCTR or siERα; (c,d) Bar plots reporting p300, GATA3 and FoxM1 fold enrichment over IgG signal at DSCAM-AS1 promoter (c) and E6-enhancer (d) by ChIP-qPCR in MCF-7 cells grown in HD and treated for 45′ with 17β-estradiol (E2) or vehicle (veh). Standard error of three biological replicates; p-value by unpaired t-test: * p < 0.05; ** p < 0.01.