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. 2018 Feb 17;19(2):598. doi: 10.3390/ijms19020598

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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HE-A, but not HE-S, reduces Aβ accumulation in APP/PS1 mice. APP/PS1 transgenic mice were orally administered with vehicle (Veh), HE-A or HE-S for 30 days (n = 8 for each group), and then the level of Aβ₁₋₄₀ and Aβ₁₋₄₂ in cortical homogenates was determined by enzyme-linked immunosorbent assay (ELISA) (A). The level of APP and C-terminal fragment (CTF) (B), and insulin-degrading enzyme (IDE) and neprilysin (NEP) (C) in homogenates were analyzed by immunoblotting. The level of IDE and NEP in wild type (WT) mice (n = 5) is also compared. Representative immunoblots are shown at the left panel. The ratio of CTF-α and −β to β-actin is presented as percentage of Veh group. The ratio of IDE to β-actin is presented as percentage of WT group. The results are the mean ± S.E.M. Significant differences between Veh group and the other groups are indicated by *, p < 0.05; **, p < 0.01.

HE-A, but not HE-S, reduces Aβ accumulation in APP/PS1 mice. APP/PS1 transgenic mice were orally administered with vehicle (Veh), HE-A or HE-S for 30 days (n = 8 for each group), and then the level of Aβ₁₋₄₀ and Aβ₁₋₄₂ in cortical homogenates was determined by enzyme-linked immunosorbent assay (ELISA) (A). The level of APP and C-terminal fragment (CTF) (B), and insulin-degrading enzyme (IDE) and neprilysin (NEP) (C) in homogenates were analyzed by immunoblotting. The level of IDE and NEP in wild type (WT) mice (n = 5) is also compared. Representative immunoblots are shown at the left panel. The ratio of CTF-α and −β to β-actin is presented as percentage of Veh group. The ratio of IDE to β-actin is presented as percentage of WT group. The results are the mean ± S.E.M. Significant differences between Veh group and the other groups are indicated by *, p < 0.05; **, p < 0.01.