Figure 4.

Reduced binding of cholera toxin on permeabilized and non-permeabilized Cln3^Δex7/8 cells. (a) Permeabilized or non-permeabilized WT and Cln3^Δex7/8 cells were incubated with FITC-coupled CTX and the fluorescence was quantitatively measured using a plate reader. Significance was assessed from three independent experiments using 2-way Anova. *** p < 0.001. (b) Permeabilized or non-permeabilized WT and Cln3^Δex7/8 cells were stained with A488-coupled CTX and imaged using a confocal fluorescence microscope. Blue staining shows the nuclei stained with DAPI. Scale bar: 20 µm. Data are representative for 3 independent experiments.