Table 1. Sequence characteristics of ITS2, matK andrbcLa in selected medicinal species of Fabaceae and Poaceae.
Fabaceae | Poaceae | |||||
---|---|---|---|---|---|---|
ITS2 | matK | rbcLa | ITS2 | matK | rbcLa | |
Universality of primers | Yes | Yes | Yes | Yes | Yes | Yes |
Percentage PCR success (%) | 85 | 71 | 100 | 100 | 100 | 100 |
Percentage sequencing success (%) | 100 | 100 | 100 | 100 | 100 | 100 |
No. of species (No. of individuals) | 7(21) | 7(21) | 7(21) | 3(9) | 3(9) | 3(9) |
No. of no sequence/singleton species | 1 | 2 | 0 | 0 | 0 | 0 |
Aligned sequence length (bp) | 360 | 844 | 553 | 365 | 772 | 553 |
Parsimony-Informative sites (bp) | 200 | 98 | 43 | 106 | 27 | 16 |
Variable sites (bp) | 209 | 99 | 44 | 106 | 27 | 17 |
Average interspecific distance (%) | 0.35 | 0.07 | 0.03 | 0.26 | 0.02 | 0.02 |
Average intraspecific distance (%) | 0.02 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |