Skip to main content
. 2018 Mar 13;6:e4499. doi: 10.7717/peerj.4499

Table 1. Sequence characteristics of ITS2, matK andrbcLa in selected medicinal species of Fabaceae and Poaceae.

Fabaceae Poaceae
ITS2 matK rbcLa ITS2 matK rbcLa
Universality of primers Yes Yes Yes Yes Yes Yes
Percentage PCR success (%) 85 71 100 100 100 100
Percentage sequencing success (%) 100 100 100 100 100 100
No. of species (No. of individuals) 7(21) 7(21) 7(21) 3(9) 3(9) 3(9)
No. of no sequence/singleton species 1 2 0 0 0 0
Aligned sequence length (bp) 360 844 553 365 772 553
Parsimony-Informative sites (bp) 200 98 43 106 27 16
Variable sites (bp) 209 99 44 106 27 17
Average interspecific distance (%) 0.35 0.07 0.03 0.26 0.02 0.02
Average intraspecific distance (%) 0.02 0.00 0.00 0.00 0.00 0.00