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. 2018 Apr;24(4):486–498. doi: 10.1261/rna.062703.117

FIGURE 3.

FIGURE 3.

BIND directly interacted with RNA containing CAG repeats and structural activity relationship study of BIND. (A–D) Isothermal titration calorimetry study of the binding affinity of BIND peptide (2 mM) to SCA2CAG22/42/55/72 RNA (4 µM). The top panel shows the raw thermogram and the bottom panel shows the binding isotherm fitted to a single-site model. (E) Amino acid sequences of TAT-BIND mutants (TAT-BIND-MT1-19). (F) Effect of TAT-BIND mutants on suppressing cell death of EGFPCAG78-expressing HEK293 cells. Data for LDH assay were normalized to the untransfected control. Each experiment was repeated at least three times and data were plotted as mean ± SEM. (*) P < 0.05, (**) P < 0.01, (****) P < 0.0001.