FIGURE 4.

The riboSNitch rs553866883 shifts the TPT1 mRNA ensemble from one predominant structure cluster into three. (A) The SHAPE-guided structural ensembles for major allele (U208), minor allele (U208C, rs11552489, nonchanger), major allele C192 all show one main structure cluster (green, I). The riboSNitch C192U (rs553866883), labeled in red, shifts the ensemble to form two new structure clusters that were previously not populated (purple, II; yellow, III). Each cluster is labeled in the ensemble plots and illustrated with the medoid structures from the group (I–III). The medoid secondary structure for the main cluster is colored by WT SHAPE reactivity while nucleotides in the alternative conformations (II and III) are colored by mutant reactivities. Points of references from the full-length structure are highlighted. (B) Comparison of normalized SHAPE data for the minor allele profile (bottom) compared to the major allele (top) around the riboSNitch.