Fig. 3. (A) The fluorescence response of the TPSNP-1 (10 μM NHS–Ad + 5 μM NpRh–Ad + 10 mM Poly-β-CD) to NaHS at different concentrations (0, 1, 2, 3, 5, 10, 20, 30, 50, 75, 100 and 150 μM). (B) The calibration curve of the TPSNP-1 to NaHS. The curve was plotted with the fluorescence intensity vs. the NaHS concentration after incubation for 40 min. (C) The linear responses at low NaHS concentrations, R = 0.997. The standard curve of the TPSNP-1 response to H2S is y = 0.30947 + 0.06531x. λex = 420 nm. (D) The TP excited fluorescence response of the TPSNP-1 to NaHS (30 μM) in PBS (pH = 7.4); the excitation wavelength was 780 nm.
