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. 2001 Sep 4;98(19):10833–10838. doi: 10.1073/pnas.191208598

Figure 4.

Figure 4

Bcl-2 inhibits SAHA-induced cell death. (A) CEM and CEM-Bcl-2 cells were treated with 0–5 μM SAHA for 24 hr or 100 ng/ml anti-Fas antibody for 12 hr; cell death was assessed by trypan blue exclusion. (B) Western blots of cytosolic extracts from CEM and CEM-Bcl-2 cells treated with or without 2.5 μM SAHA were probed with mAbs to cytochrome c (c) and α-tubulin (t). In some wells, CEM cells were pretreated with 40 μM ZVAD-fmk (lanes 4 and 7) or ZFA-fmk (lanes 5 and 8) for 1 hr and then cultured for 24 hr with 2.5 μM SAHA.