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. 2018 Mar 15;7:2. doi: 10.1186/s13630-018-0056-1

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Odour detection in OSNs of wild-type Tg(elavl3:GCaMP5) at 2.5 dpf. a Frames of food odour response time course displayed as RAW image (top row) and DFF (change in fluorescence over baseline fluorescence; ΔF/F, DFF; bottom row). b Example traces of individual OSNs responses in time after addition of food odour, dashed line represents odour arrival at olfactory epithelium and solid line represents duration of odour delivery. Coloured lines represent individual example OSN responses, thick black line is mean of all OSNs (N = 382 OSNs). Data acquisition speed was 2 Hz. c Mean OSN-response amplitude to different odour classes used in this study compared to the water control (N = 4 fish, 382 OSNs, ***P < 0.001, Mann–Whitney U test). Bars represent mean and SEM. Scale bar is 10 µm