Fig. 4.

Effect of ift172-deficiency on cilia-dependent olfactory sensory neuron signalling in 2.5-dpf-old zebrafish. a Significant change in percentage of responding cells per embryo to nucleotides in Tg(elavl3:GCaMP5) ift172 MO OSNs compared to control MO OSNs (N = 517 OSNs, 5 fish for control MO and N = 495 OSNs, 6 fish for ift172 MO). b Significantly reduced response amplitude to bile acids and food odour in Tg(elavl3:GCaMP5) ift172 MO responding OSNs compared to control MO responding OSNs. c No difference in Omp-positive OSNs between control and ift172 MO Tg(omp:gal4,UAS:YPF) fish. d Quantification of C (N = 3 control MO, N = 4 ift172 MO, P = 0.33). e No difference in number of GCaMP5-positive OSNs per embryo (P = 0.16). Bars represent mean and SEM (*P < 0.05, **P < 0.01, ***P < 0.001 Mann–Whitney U test for a, b, Student’s t test for d, e). Scale bar is 10 µm