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. 2018 Mar 12;13:1457–1470. doi: 10.2147/IJN.S155814

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© 2018 Shi et al. This work is published and licensed by Dove Medical Press Limited

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Effects of HAPs on ALP activity and extracellular calcium deposition of hWJ-MSCs. (A) ALP activity of hWJ-MSCs cultured with HAPs for 7 days. (B) The extracellular calcium deposition was visualized by Alizarin Red S staining after cells were cultured with HAPs for 14 days (middle panel). Cells without HAPs treatment were set as the control group (bottom panel).

Abbreviations: HAPs, hydroxyapatite particles; ALP, alkaline phosphatase; hWJ-MSCs, human umbilical cord Wharton’s jelly-derived mesenchymal stem cells; np20, hydroxyapatite nanoparticles 20 nm in diameter; np80, hydroxyapatite nanoparticles 80 nm in diameter; m-HAP, micro-sized HAP particles; HAP, hydroxyapatite.