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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Biologicals. 2017 Sep 29;50:27–34. doi: 10.1016/j.biologicals.2017.09.005

Fig. 3. SEC analysis.

Fig. 3

A) Lower trace: Size exclusion chromatograph (SEC) of freshly prepared Fab fragment (detector set at λabs = 280 nm) that displays a single eluent, corresponding to a ≈50 kDa product. Upper trace: SEC of the Fab fragment after shaking for 4 h at 23 °C on the platform of a vortex mixer (See text.). B) The deconvoluted electrospray quadrupole time-of-flight (ESI-Q-TOF) mass spectrum from effluent at the signal maximum of the SEC chromatography trace. Thedeconvoluted peak at 47,628 (±5) m/z, indicates a measured molecular weight for the Fab fragment of 4762 (±5) g/mol.