| PBS | 50 ml | |
| EDTA | 5 mM | 0.5 ml × 0.5 M (pH 7.4) |
| EGTA | 5 mM | 0.5 ml × 0.5 M (pH 7.4) |
| NaPyrophophate | 10 mM | 0.223 g |
| NaF | 50 mM | 0.1 g |
| NaVO3 | 1 mM | 0.5 ml × 100 mM |
| 1% Triton | ||
| 1 tablet Protease inhibitor |
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