Fig. 4.

The position of the arginine residue in the HIV-1 Env TMD does not affect its intracellular targeting. a In the T-E26 chimera, an arginine is present in the Env TMD at position 14. A series of chimeric proteins where the arginine was moved to other positions were constructed (T-E41 to T-E46) (see also Table 1). Positions within the sequence are numbered from the luminal to the cytoplasmic end of the TMD. b The fraction of each chimeric protein present at the cell surface was determined as described in the legend to Fig. 2. Statistical analysis revealed no significant differences from T-H0 (n = 3; one-way analysis of variance: p = 0.77)