Step	Metafectamine SI	RNAiMAX
1	Dilute Metafectamine buffer with H2O to make 1x buffer	Dilute RNAiMAX (0.3 μl) with 5 μl Opti-MEM medium
2 (see Note 1)	Mix the following components: siRNA (5 μM stock): 1.2 μl 1x Metafectamine SI buffer: 15 μl Metafectamine SI: 0.33 μl	Dilute siRNA (1.2 μl stock (5 μM)) with 5 μl Opti-MEM medium
3		Mix RNAiMAX and siRNA
4	Incubate for 15 min at room temperature	Incubate for 5 min at room temperature