1	Mix the following components: NPY-Venus lenti construct: 20 μg Envelop plasmid pMD2.G: 6 μg Packaging plasmid psPAX2: 15 μg CaCl2 (2.5 M) (see Recipes): 50 μl Add H2O to bring total volume to 500 μl	500 μl 2x HBeS (see Recipes)
2	Mix plasmid mixture dropwise with HBeS
3	Incubate at room temperature for 20 min
4	Add plasmid mixture (1 ml) dropwise to HEK cells