Figure 1.

In vivo experimental set up. A, Schema of experimental set up. Animals have a tunneled central venous catheter inserted 24 hours prior to induction of sepsis. At 2 hours, IV fluid resuscitation is commenced. Animals are anesthetized and laparotomy performed at 6 or 24 hours for measurement of cardiac output, renal blood flow, renal cortical oxygen tension and renal vein oxygen tension, and renal tissue sampling. B, Flow probe measuring renal blood flow. Oxygen sensor inserted into lower pole of renal cortex. C, Oxygen sensor inserted into the mid-pole of the renal cortex.