Fig. 2.

Viperin promotes the systemic IFNα response. a WT and viperin^−/− mice were infected intraperitoneally with 10⁴ FFU of LGTV. Serum samples were collected at indicated time points (n = 5). The IFNα protein level was measured by ELISA. b, c WT and viperin^−/− mice were infected intraperitoneally with 10⁴ FFU of LGTV. The olfactory bulb was collected at indicated time points (n = 5), and IFNβ and IFNα₂ transcripts were detected using qPCR. Expression levels were normalized to the endogenous GAPDH expression and depicted as fold induction over uninfected. Each data point represents an individual mouse. Bars indicated mean values. Asterisks indicate statistical significance calculated by the Mann-Whitney test (*P < 0.05, **P < 0.005; ***P < 0.001)